COVID-19 Test Equipment

  • Nucleic Acid Detection (PCR) Kit for Detection of 2019-nCoV
Nucleic Acid Detection (PCR) Kit for Detection of 2019-nCoV

Nucleic Acid Detection (PCR) Kit for Detection of 2019-nCoV

  • Product description: The kit is a Nucleic Acid Detection PCR to qualitatively detect ORF1ab, N and E genes nucleic acid of 2019-nCoV in throat swab, sputum in patients with respiratory infection symptoms and close cont
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【Product name】
Nucleic Acid Detection PCR Kit for Detection of 2019-nCoV
【Packing specification】48 tests/kit
【Catalogue number】 CT8223-48T
【Intended use】
The kit is a TaqMan probe real-time fluorescent PCR to qualitatively detect ORF1ab,
N and E genes nucleic acid of 2019-nCoV in throat swab, sputum in patients with
respiratory infection symptoms and close contacts.
The test results are for clinical reference only and cannot be used as a basis for
confirming or eliminating cases. Operators should have experience of professional
training in gene amplification or molecular biology method testing, and have relevant
qualifications for laboratory operations. The laboratory should have reasonable
biological safety precautions and protective procedures.
【Test Principle】
In this kit, primers and probes of real-time fluorescent PCR technology are
designed to the conserved and specific regions of the ORF1ab, N and E genes of
2019-nCoV, respectively. During PCR amplification, the probe binds to the template,
and the 5'-end reporter group of the probe is cleaved by the Taq enzyme (5' → 3'
exonuclease activity), thereby moving away from the quenching group to generate a
fluorescent signal. The real-time amplification curve is automatically plotted based on
the detected fluorescence signal, and the sample Ct value is calculated. FAM and VIC
fluorophores are labeled to ORF1ab gene and N gene probes, respectively in reaction
solution A, and FAM fluorophore is labeled to E gene probe in reaction solution B. By
using one test, qualitative detection of the above three genes of 2019-nCoV can be
performed simultaneously.
The kit is provided with an internal control(IC), which is a pathogen-unrelated
gene fragment, and is labeled with a Cy5 fluorescent group

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